Radiology, Vol 149, 563-566, Copyright © 1983 by Radiological Society of North America
Neutrophil labeling with indium-111: tropolone vs. oxine
KP Gunter, JN Lukens, JA Clanton, PJ Morris, RL Janco and D English
This study was undertaken to compare tropolone with oxine (8-hydroxy-
quinoline) for labeling human neutrophils with In-111. Exposure of
neutrophils to tropolone at concentrations required for efficient labeling
resulted in a marked impairment of chemotaxis. In contrast, no impairment
of neutrophil chemotaxis was observed using In-111 oxine. Labeling
efficiencies obtained with In-111 tropolone under optimal conditions were
consistently less than those obtained with In-111 oxine. We evaluated cells
labeled by the two methods using chemotaxis radioassay to assess the
chemotatic potential of labeled cells. The results led to the conclusion
that the oxine technique is preferable to tropolone for labeling human
neutrophils with In-111.
