Radiology, Vol 171, 653-656, Copyright © 1989 by Radiological Society of North America
Mesenteric vascular occlusion: a new diagnostic method using a radiolabeled monoclonal antibody reactive with platelets
ZH Oster, P Som and PO Zamora
Department of Radiology, State University of New York, Stony Brook 11794-8460.
A new method for diagnosing mesenteric vaso-occlusive bowel disease with
the use of radioimmunoscintigraphy was developed and tested in experimental
models of arterial and venous disease, as well as in a model simulating
bowel strangulation. The method involves the use of a monoclonal antibody
fragment mixture that binds to platelets. The antibody was labeled with
technetium-99m, and imaging was performed with a gamma camera in the planar
and single photon emission computed tomography modes. This method allowed
visualization of areas of ischemia of 1-6 hours duration in bowel loops in
19 dogs 90-180 minutes after injection of the radiolabeled antibody. No
bowel radioactivity accumulation occurred in dogs that underwent the same
surgical procedure but were given a nonspecific Tc-99m-labeled antibody or
in normal dogs given the specific antibody. It appears that the
radiolabeled antibody used, which has higher reactivity with human
platelets than with dog platelets, will be a good agent for noninvasive
diagnosis of mesenteric vaso-occlusive disease in humans. It may also play
a role in the intraoperative determination of the extent and location of
ischemic bowel segments.
