Radiology, Vol 196, 267-269, Copyright © 1995 by Radiological Society of North America
Evaluation of 2-(3-aminobenzamido)-2-deoxy-D-glucose as a radiosensitizer
SL Winer, R Bases, I Lalezari, M Brownlee and WA Franklin
Department of Radiation Oncology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
PURPOSE: 3-Aminobenzamide (3-AB), an inhibitor of poly(adenosine
diphosphate [ADP]-ribose) synthetase, functions as a radiosensitizer in
several human tumor cell lines. 2-(3-AB)-2-deoxy-D-glucose (3-AB-G) was
designed to increase preferentially the intracellular concentration of the
drug in tumor cells. Both the toxicity and effectiveness of 3-AB-G as a
radiosensitizer were determined. MATERIALS AND METHODS: The toxicity of
3-AB-G was measured in HeLa and Chinese hamster ovary cells. The
radiosensitizing effect of 3-AB-G was determined for both cell lines.
RESULTS: 3-AB-G was not toxic to cells at concentrations of 10 mmol/L or
less. 3-AB-G did not alter cell survival after irradiation. CONCLUSION:
3-AB-G was not an effective radiosensitizer for the cells tested. Coupling
2-deoxyglucose to 3-AB may block the uptake of the inhibitor into the cell
by altering the ability of the receptor to recognize the molecule or may
interfere with the specificity of the inhibitor for poly(ADP-ribose)
synthetase.
