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Hepatic Colon Cancer Metastases in Mice: Dynamic in Vivo Correlation with Hypoechoic Rims Visible at US¹

¹ From the Departments of Radiology (J.B.K., I.N., O.C., R.A.K.) and Cancer Biology (P.T.), Beth Israel Deaconess Medical Center-West, One Deaconess Rd, Boston, MA 02215. From the 1998 RSNA scientific assembly. Received May 19, 1999; revision requested July 2; revision received August 12; accepted September 30. J.B.K. supported in part by the RSNA Research and Education Foundation as a General Electric Medical Systems/RSNA Scholar, a Biomedical Research Support Grant from Beth Israel Deaconess Medical Center, and a grant from the Beth Israel Deaconess Medical Center Radiology Foundation. Address correspondence to J.B.K. (e-mail: jkruskal@caregroup.harvard.edu).

PURPOSE: To use videomicroscopy of tumor-bearing livers of live mice to depict tumors directly to determine the exact nature of rims seen on corresponding ultrasonographic (US) scans.

MATERIALS AND METHODS: Seventy-six hepatic colorectal cancer metastases were studied in exteriorized livers of 18 mice by using intravital microscopy, US, and histologic examination of the same tumors.

RESULTS: Hypoechoic rims correlated with distended sinusoidal spaces in vivo. These spaces surrounded only locally invasive tumors (mean diameter, 0.85 mm) that had obstructed the supplying terminal portal venules. These spaces, containing adherent leukocytes and tumor cells, gave rise to new tumor vasculature. Results of histologic examination of rims (portal inflammation, congested or compressed sinusoids, cell atrophy) correlated with leukocyte endothelial adherence, occluded sinusoids, and new vessel formation in vivo.

CONCLUSION: Unlike results from previous studies, dynamic in vivo observations of peritumoral rims demonstrated distended sinusoidal spaces giving rise to new tumor-penetrating vessels. These sinusoids arose around locally invasive tumors and were associated with more advanced intrahepatic disease. These dynamic observations provide a pathophysiologic explanation for previous histologic correlates of peritumoral rims.

Index terms: Blood, flow dynamics, 95.92 • Colon, neoplasms, 75.32 • Liver neoplasms, blood supply, 95.92 • Liver neoplasms, secondary, 761.1267, 761.337 • Liver neoplasms, US, 761.12981, 761.12983 • Ultrasound (US), comparative studies

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