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(Radiology. 2001;218:881-885.)
© RSNA, 2001

Technical Developments

Stereotactic Core Biopsy Breast and Blood Cell By-products: A Source of Material for Molecular Genetics Research—Initial Experience1

Paul C. Stomper, MD, Carleton C. Stewart, PhD and Daniel L. Stoler, PhD

1 From the Departments of Diagnostic Imaging (P.C.S.), Flow Cytometry (C.C.S.), and Experimental Pathology (D.L.S.), Roswell Park Cancer Institute, School of Medicine and Biomedical Sciences, SUNY, Elm and Carlton Sts, Buffalo, NY 14263. Received November 18, 1999; revision requested December 28; final revision received July 11, 2000; accepted August 15. Address correspondence to P.C.S. (e-mail: Paul.Stomper@roswellpark.org).

Stereotactic core biopsy washings and blood drop samples, routinely discarded by-products, provide satisfactory fresh cellular material for flow cytometry and molecular genetics microsatellite polymerase chain reaction (PCR) analysis for detection of loss of DNA alleles (loss of heterozygosity). Cytokeratin-positive (epithelial) cells from the core biopsy washings were sorted by means of flow cytometry prior to PCR analysis. DNA allele loss was detected in benign breast epithelial cells in three (20%) of 15 patients.

Index terms: Breast, biopsy, 00.1267 • Breast, diseases, 00.72 • Breast neoplasms, 00.30 • Genes and genetics • Molecular analysis • Specimens




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D. L. Stoler, C. C. Stewart, and P. C. Stomper
Breast Epithelium Procurement from Stereotactic Core Biopsy Washings: Flow Cytometry-sorted Cell Count Analysis
Clin. Cancer Res., February 1, 2002; 8(2): 428 - 432.
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