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Effects of Exposure of CHO-K1 Cells to a 10-T Static Magnetic Field¹

¹ From the Department of Radiation Genetics, Graduate School of Medicine, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. Received July 30, 2001; revision requested September 9; final revision received March 18, 2002; accepted March 26. Supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by a grant-in-aid from the Research for the Future Program, Japanese Society for the Promotion of Science. Address correspondence to J.M. (e-mail: miyakosh@mfour.med.kyoto-u.ac.jp).

PURPOSE: To evaluate whether exposure to strong static magnetic fields (SMFs), of up to 10 T, affects the growth and cycle distribution of and the micronucleus formation in monolayered Chinese hamster ovary CHO-K1 cells.

MATERIALS AND METHODS: The authors developed a system to expose cultured cells to strong SMFs immediately after the cells are seeded. Cell growth rate was evaluated according to cell number count. Cell cycle distribution experiments were performed by using flow cytometric analysis. In these experiments, the cells were exposed to SMFs for up to 4 days. The frequency of micronucleus formation with only SMF exposure at x-ray irradiation was analyzed at microscopic observation.

RESULTS: Long-term exposure to a 10-T SMF for up to 4 days did not affect cell growth rate or cell cycle distribution. Exposure to SMFs alone did not affect micronucleus frequency. In x-ray–irradiated cells, exposure to a 1-T SMF did not affect micronucleus frequency, but exposure to a 10-T SMF resulted in a significant (P < .05) increase in micronucleus frequency.

CONCLUSION: Strong (10-T) SMFs have no effect on cell growth, cell cycle distribution, or micronucleus frequency, but they may cause an increase in the micronucleus formation induced by 4-Gy x rays.

© RSNA, 2002

Index terms: Experimental study • Magnetic resonance (MR), experimental studies, _**.1214³ • Magnetic resonance (MR), high-field-strength imaging, _**.1214 • Magnetic resonance (MR), safety, _**.1214