| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Experimental Studies |
1 From the Experimental Neuroimaging Section, Laboratory of Diagnostic Radiology Research, Clinical Center, National Institutes of Health, Bldg 10, Rm B1N256, 10 Center Dr, MSC 1074, Bethesda, MD 20892-1074. Received May 30, 2002; revision requested July 29; final revision received October 9; accepted November 5. Address correspondence to J.A.F. (e-mail: jafrank@helix.nih.gov).
PURPOSE: To label mammalian and stem cells by combining commercially available transfection agents (TAs) with superparamagnetic iron oxide (SPIO) magnetic resonance (MR) imaging contrast agents.
MATERIALS AND METHODS: Three TAs were incubated with ferumoxides and MION-46L in cell culture medium at various concentrations. Human mesenchymal stem cells, mouse lymphocytes, rat oligodendrocyte progenitor CG-4 cells, and human cervical carcinoma cells were incubated 2–48 hours with 25 µg of iron per milliliter of combined TAs and SPIO. Cellular labeling was evaluated with T2 relaxometry, MR imaging of labeled cell suspensions, and Prussian blue staining for iron assessment. Proliferation and viability of mesenchymal stem cells and human cervical carcinoma cells labeled with a combination of TAs and ferumoxides were evaluated.
RESULTS: When ferumoxides-TA or MION-46L–TA was used, intracytoplasmic particles stained with Prussian blue stain were detected for all cell lines with a labeling efficiency of nearly 100%. Limited or no uptake was observed for cells incubated with ferumoxides or MION-46L alone. For TA-SPIO–labeled cells, MR images and relaxometry findings showed a 50%–90% decrease in signal intensity and a more than 40-fold increase in T2s. Cell viability varied from 103.7% ± 9 to 123.0% ± 9 compared with control cell viability at 9 days, and cell proliferation was not affected by endosomal incorporation of SPIO nanoparticles. Iron concentrations varied with ferumoxides-TA combinations and cells with a maximum of 30.1 pg ± 3.7 of iron per cell for labeled mesenchymal stem cells.
CONCLUSION: Magnetic labeling of mammalian cells with use of ferumoxides and TAs is possible and may enable cellular MR imaging and tracking in experimental and clinical settings.
© RSNA, 2003
Index terms: Cell labeling • Experimental study • Iron • Magnetic resonance (MR), experimental studies • Stem cells
This article has been cited by other articles:
|
|
 |
|
  M. Klabusay, P. Scheer, M. Doubek, K. Rehakova, P. Coupek, and D. Horky Retention of Nanoparticles-Labeled Bone Marrow Mononuclear Cells in the Isolated Ex Vivo Perfused Heart After Myocardial Infarction in Animal Model Experimental Biology and Medicine, February 1, 2009; 234(2): 222 - 231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nahrendorf, D. E. Sosnovik, B. A. French, F. K. Swirski, F. Bengel, M. M. Sadeghi, J. R. Lindner, J. C. Wu, D. L. Kraitchman, Z. A. Fayad, et al. Multimodality Cardiovascular Molecular Imaging, Part II Circ Cardiovasc Imaging, January 1, 2009; 2(1): 56 - 70. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Jing, N. Mal, P. S. Williams, M. Mayorga, M. S. Penn, J. J. Chalmers, and M. Zborowski Quantitative intracellular magnetic nanoparticle uptake measured by live cell magnetophoresis FASEB J, December 1, 2008; 22(12): 4239 - 4247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Terrovitis, K. F. Kwok, R. Lautamaki, J. M. Engles, A. S. Barth, E. Kizana, J. Miake, M. K. Leppo, J. Fox, J. Seidel, et al. Ectopic Expression of the Sodium-Iodide Symporter Enables Imaging of Transplanted Cardiac Stem Cells In Vivo by Single-Photon Emission Computed Tomography or Positron Emission Tomography J. Am. Coll. Cardiol., November 11, 2008; 52(20): 1652 - 1660. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Sadan, N. Shemesh, R. Barzilay, M. Bahat-Stromza, E. Melamed, Y. Cohen, and D. Offen Migration of Neurotrophic Factors-Secreting Mesenchymal Stem Cells Toward a Quinolinic Acid Lesion as Viewed by Magnetic Resonance Imaging Stem Cells, October 1, 2008; 26(10): 2542 - 2551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. H. Schuleri, L. C. Amado, A. J. Boyle, M. Centola, A. P. Saliaris, M. R. Gutman, K. E. Hatzistergos, B. N. Oskouei, J. M. Zimmet, R. G. Young, et al. Early improvement in cardiac tissue perfusion due to mesenchymal stem cells Am J Physiol Heart Circ Physiol, May 1, 2008; 294(5): H2002 - H2011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Terrovitis, M. Stuber, A. Youssef, S. Preece, M. Leppo, E. Kizana, M. Schar, G. Gerstenblith, R. G. Weiss, E. Marban, et al. Magnetic Resonance Imaging Overestimates Ferumoxide-Labeled Stem Cell Survival After Transplantation in the Heart Circulation, March 25, 2008; 117(12): 1555 - 1562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Neri, C. Maderna, C. Cavazzin, V. Deidda-Vigoriti, L. S. Politi, G. Scotti, P. Marzola, A. Sbarbati, A. L. Vescovi, and A. Gritti Efficient In Vitro Labeling of Human Neural Precursor Cells with Superparamagnetic Iron Oxide Particles: Relevance for In Vivo Cell Tracking Stem Cells, February 1, 2008; 26(2): 505 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Maxwell, J. Bonde, D. A. Hess, S. A. Hohm, R. Lahey, P. Zhou, M. H. Creer, D. Piwnica-Worms, and J. A. Nolta Fluorophore-Conjugated Iron Oxide Nanoparticle Labeling and Analysis of Engrafting Human Hematopoietic Stem Cells Stem Cells, February 1, 2008; 26(2): 517 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kuhlpeter, H. Dahnke, L. Matuszewski, T. Persigehl, A. von Wallbrunn, T. Allkemper, W. L. Heindel, T. Schaeffter, and C. Bremer R2 and R2* Mapping for Sensing Cell-bound Superparamagnetic Nanoparticles: In Vitro and Murine in Vivo Testing Radiology, November 1, 2007; 245(2): 449 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ju, G.-J. Teng, H. Lu, Y. Zhang, A. Zhang, F. Chen, and Y. Ni In Vivo MR Tracking of Mesenchymal Stem Cells in Rat Liver after Intrasplenic Transplantation Radiology, October 1, 2007; 245(1): 206 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Politi, M. Bacigaluppi, E. Brambilla, M. Cadioli, A. Falini, G. Comi, G. Scotti, G. Martino, and S. Pluchino Magnetic Resonance-Based Tracking and Quantification of Intravenously Injected Neural Stem Cell Accumulation in the Brains of Mice with Experimental Multiple Sclerosis Stem Cells, October 1, 2007; 25(10): 2583 - 2592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Guzman, N. Uchida, T. M. Bliss, D. He, K. K. Christopherson, D. Stellwagen, A. Capela, J. Greve, R. C. Malenka, M. E. Moseley, et al. Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI PNAS, June 12, 2007; 104(24): 10211 - 10216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Partlow, J. Chen, J. A. Brant, A. M. Neubauer, T. E. Meyerrose, M. H. Creer, J. A. Nolta, S. D. Caruthers, G. M. Lanza, and S. A. Wickline 19F magnetic resonance imaging for stem/progenitor cell tracking with multiple unique perfluorocarbon nanobeacons FASEB J, June 1, 2007; 21(8): 1647 - 1654. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. E. Rice, E. W. Hsu, H. Sheng, D. A. Evenson, A. J. Freemerman, K. M. Safford, J. M. Provenzale, D. S. Warner, and G. A. Johnson Superparamagnetic Iron Oxide Labeling and Transplantation of Adipose-Derived Stem Cells in Middle Cerebral Artery Occlusion-Injured Mice Am. J. Roentgenol., April 1, 2007; 188(4): 1101 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Wisel, S. M. Chacko, M. L. Kuppusamy, R. P. Pandian, M. Khan, V. K. Kutala, R. W. Burry, B. Sun, P. Kwiatkowski, and P. Kuppusamy Labeling of skeletal myoblasts with a novel oxygen-sensing spin probe for noninvasive monitoring of in situ oxygenation and cell therapy in heart Am J Physiol Heart Circ Physiol, March 1, 2007; 292(3): H1254 - H1261. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Vemulapalli, S. D. Metzler, G. Akabani, N. A. Petry, N. J. Niehaus, X. Liu, N. H. Patil, K. L. Greer, R. J. Jaszczak, R. E. Coleman, et al. Cell Therapy in Murine Atherosclerosis: In Vivo Imaging with High-Resolution Helical SPECT Radiology, January 1, 2007; 242(1): 198 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Zhou, P. D. Acton, and V. A. Ferrari Imaging Stem Cells Implanted in Infarcted Myocardium J. Am. Coll. Cardiol., November 21, 2006; 48(10): 2094 - 2106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Tai, P. Foster, A. Rosales, B. Feng, C. Hasilo, V. Martinez, S. Ramadan, J. Snir, C.W. J. Melling, S. Dhanvantari, et al. Imaging Islets Labeled With Magnetic Nanoparticles at 1.5 Tesla Diabetes, November 1, 2006; 55(11): 2931 - 2938. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Wickline, A. M. Neubauer, P. Winter, S. Caruthers, and G. Lanza Applications of Nanotechnology to Atherosclerosis, Thrombosis, and Vascular Biology Arterioscler. Thromb. Vasc. Biol., March 1, 2006; 26(3): 435 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. L. Wu, Q. Ye, L. M. Foley, T. K. Hitchens, K. Sato, J. B. Williams, and C. Ho In situ labeling of immune cells with iron oxide particles: An approach to detect organ rejection by cellular MRI PNAS, February 7, 2006; 103(6): 1852 - 1857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Provenzale Good Things Come in Small Packages: A Review of the Proceedings of the 2005 Academy of Molecular Imaging Meeting Am. J. Roentgenol., February 1, 2006; 186(2): 291 - 296. [Full Text] [PDF]
|
|
|
|
|
|
D. L. Kraitchman, M. Tatsumi, W. D. Gilson, T. Ishimori, D. Kedziorek, P. Walczak, W. P. Segars, H. H. Chen, D. Fritzges, I. Izbudak, et al. Dynamic Imaging of Allogeneic Mesenchymal Stem Cells Trafficking to Myocardial Infarction Circulation, September 6, 2005; 112(10): 1451 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Riviere, F. P. Boudghene, F. Gazeau, J. Roger, J.-N. Pons, J.-P. Laissy, E. Allaire, J.-B. Michel, D. Letourneur, and J.-F. Deux Iron Oxide Nanoparticle-labeled Rat Smooth Muscle Cells: Cardiac MR Imaging for Cell Graft Monitoring and Quantitation Radiology, June 1, 2005; 235(3): 959 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Zhou, D. H. Thomas, H. Qiao, H. S. Bal, S.-R. Choi, A. Alavi, V. A. Ferrari, H. F. Kung, and P. D. Acton In Vivo Detection of Stem Cells Grafted in Infarcted Rat Myocardium J. Nucl. Med., May 1, 2005; 46(5): 816 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. T. Yocum, L. B. Wilson, P. Ashari, E. K. Jordan, J. A. Frank, and A. S. Arbab Effect of Human Stem Cells Labeled with Ferumoxides- Poly-L-lysine on Hematologic and Biochemical Measurements in Rats Radiology, May 1, 2005; 235(2): 547 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Matuszewski, T. Persigehl, A. Wall, W. Schwindt, B. Tombach, M. Fobker, C. Poremba, W. Ebert, W. Heindel, and C. Bremer Cell Tagging with Clinically Approved Iron Oxides: Feasibility and Effect of Lipofection, Particle Size, and Surface Coating on Labeling Efficiency Radiology, April 1, 2005; 235(1): 155 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. E. Daldrup-Link, M. Rudelius, G. Piontek, S. Metz, R. Brauer, G. Debus, C. Corot, J. Schlegel, T. M. Link, C. Peschel, et al. Migration of Iron Oxide-labeled Human Hematopoietic Progenitor Cells in a Mouse Model: In Vivo Monitoring with 1.5-T MR Imaging Equipment Radiology, January 1, 2005; 234(1): 197 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Anderson, J. Glod, A. S. Arbab, M. Noel, P. Ashari, H. A. Fine, and J. A. Frank Noninvasive MR imaging of magnetically labeled stem cells to directly identify neovasculature in a glioma model Blood, January 1, 2005; 105(1): 420 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Homma, H. Yoshikawa, M. Takeno, M. S. Kurokawa, C. Masuda, E. Takada, K. Tsubota, S. Ueno, and N. Suzuki Induction of Epithelial Progenitors In Vitro from Mouse Embryonic Stem Cells and Application for Reconstruction of Damaged Cornea in Mice Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4320 - 4326. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Mahmood Can MR Imaging Be Used to Track Delivery of Intravascularly Administered Stem Cells? Radiology, December 1, 2004; 233(3): 625 - 626. [Full Text] [PDF]
|
|
|
|
|
|
C. Bos, Y. Delmas, A. Desmouliere, A. Solanilla, O. Hauger, C. Grosset, I. Dubus, Z. Ivanovic, J. Rosenbaum, P. Charbord, et al. In Vivo MR Imaging of Intravascularly Injected Magnetically Labeled Mesenchymal Stem Cells in Rat Kidney and Liver Radiology, December 1, 2004; 233(3): 781 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. V. Frangioni and R. J. Hajjar In Vivo Tracking of Stem Cells for Clinical Trials in Cardiovascular Disease Circulation, November 23, 2004; 110(21): 3378 - 3383. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Gibbons and P. A. Araoz The year in cardiac imaging J. Am. Coll. Cardiol., November 16, 2004; 44(10): 1937 - 1944. [Full Text] [PDF]
|
|
|
|
|
|
J. W. M. Bulte, D. L. Kraitchman, A. M. Mackay, M. F. Pittenger, A. S. Arbab, G. T. Yocum, H. Kalish, E. K. Jordan, S. A. Anderson, A. Y. Khakoo, et al. Chondrogenic differentiation of mesenchymal stem cells is inhibited after magnetic labeling with ferumoxides Blood, November 15, 2004; 104(10): 3410 - 3413. [Full Text] [PDF]
|
|
|
|
|
|
A. S. Arbab, G. T. Yocum, H. Kalish, E. K. Jordan, S. A. Anderson, A. Y. Khakoo, E. J. Read, and J. A. Frank Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI Blood, August 15, 2004; 104(4): 1217 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Warach and J.-C. Baron Neuroimaging Stroke, February 1, 2004; 35(2): 351 - 353. [Full Text] [PDF]
|
|
|
|
|
|
E. C. Unger How Can Superparamagnetic Iron Oxides Be Used to Monitor Disease and Treatment? Radiology, December 1, 2003; 229(3): 615 - 616. [Full Text] [PDF]
|
|
|
|
|
|
A. S. Arbab, L. A. Bashaw, B. R. Miller, E. K. Jordan, B. K. Lewis, H. Kalish, and J. A. Frank Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging Radiology, December 1, 2003; 229(3): 838 - 846. [Abstract] [Full Text] [PDF]
|
|
