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DOI: 10.1148/radiol.2282020539
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(Radiology 2003;228:555-559.)
© RSNA, 2003

Technical Developments

In Vivo US Monitoring of Catheter-based Vascular Delivery of Gene Microspheres in Pigs: Feasibility1

Xiangying Du, MD, Yuesong Yang, MD, PhD, Catherine Le Visage, PhD, Hunter H. Chen, BS, Robert DeJong, BS, Bensheng Qiu, PhD, Danming Wang, MD, Kam W. Leong, PhD, Ulrike M. Hamper, MD, MBA and Xiaoming Yang, MD, PhD

1 From the Departments of Radiology (X.D., Y.Y., R.D., B.Q., D.W., U.M.H., X.Y.) and Biomedical Engineering (C.L.V., H.H.C., K.W.L.), Johns Hopkins University School of Medicine, 720 Rutland Ave, Traylor Bldg, Rm 330, Baltimore, MD 21205. Received May 9, 2002; revision requested July 11; final revision received October 22; accepted December 10. Supported in part by a Johns Hopkins Radiology Molecular Imaging grant and National Institutes of Health grants R01 HL66187 and R01 67195. Address correspondence to X.Y. (e-mail: xyang@mri.jhu.edu).

In this study, the authors tested the feasibility of using ultrasonography (US) to monitor catheter-based vascular gene microsphere delivery. Polymeric biodegradable microspheres (mean diameter, 5 µm) were prepared by using a double-emulsion technique to encapsulate DNA-plasmid–encoding green fluorescent protein (GFP) genes. With use of gene-delivery catheters, GFP microspheres were locally delivered into the left femoral arterial walls of six pigs; the contralateral arteries were not infused with microspheres and thus served as negative control vessels. The delivery procedures were monitored with high-frequency (8–15-MHz) transducer US. The effectiveness of monitoring with US was compared with the effectiveness of monitoring with immunohistochemical anti-GFP staining. A highly echogenic "star burst" sign around the entire vessel wall was seen at US and correlated with immunohistochemical findings that showed the destination of the gene microspheres. Study results demonstrate the potential of US for monitoring catheter-based vascular gene microsphere delivery in vivo.

© RSNA, 2003

Index terms: Animals • Genes and genetics • Microspheres • Ultrasound (US), experimental studies, 92.12981, 92.12982, 92.12983, 92.12988, 92.12989




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