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Molecular Imaging |
1 From Schering, Research Laboratories, Müllerstrasse 178, D-13342 Berlin, Germany. Received March 19, 2003; revision requested May 27; final revision received September 2; accepted September 29. Address correspondence to P.H. (e-mail: peter.hauff@schering.de).
PURPOSE: To evaluate the feasibility of using intravenously administered L-selectin ligand-specific polymer-stabilized air-filled microparticles (MPs) for active targeting of peripheral lymph nodes under normal conditions in animal models.
MATERIALS AND METHODS: L-selectin ligand-specific MPs and two control substances (immunoglobulin M–isotype MPs and native MPs) were each administered in three conscious mice as a single intravenous bolus injection (1.4 x 107 MPs/kg). All mice were sacrificed 30 minutes after administration. Lymph nodes (cervical, inguinal, axillary, popliteal, mesenteric), spleen (positive control), and kidney (blood pool control) were removed and examined for MP-related stimulated acoustic emission (SAE) signals by using harmonic color Doppler ultrasonography (US) in a tank containing degassed water. A second experiment was performed in six anesthetized beagle dogs by using the same MP formulation. Each of the MP formulations was administered in two anesthetized dogs as a single intravenous bolus injection (1 x 107 MPs/kg). The popliteal lymph nodes, spleen (positive control), and kidney (blood pool control) were examined in vivo with US for MP-related SAE signals 30 minutes after administration. Fisher exact test for the one-side alternative was used for mouse data analysis.
RESULTS: The lymph nodes of all mice (P = .05) and the popliteal lymph nodes of both dogs treated with L-selectin ligand-specific MPs showed clear MP-related SAE signals, whereas the lymph nodes of all mice and the popliteal lymph nodes of four dogs that received the control substances did not show any SAE signals.
CONCLUSION: Use of an intravenously administered L-selectin ligand-specific US contrast agent is feasible for active lymph node targeting in mice and dogs.
© RSNA, 2004
Index terms: Animals • Contrast media, experimental studies • Lymphatic system, US, 998.12983, 998.12988 • Molecular analysis • Ultrasound (US), experimental studies
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