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Published online before print June 21, 2005, 10.1148/radiol.2362040603
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(Radiology 2005;236:579-587.)
© RSNA, 2005

Molecular Imaging

T-Cell Homing to the Pancreas in Autoimmune Mouse Models of Diabetes: In Vivo MR Imaging1

Claire Billotey, MD, PhD, Caroline Aspord, PhD, Olivier Beuf, PhD, Eliane Piaggio, PhD, Florence Gazeau, PhD, Marc F. Janier, MD, PhD and Charles Thivolet, MD, PhD

1 From the Animage-CREATIS, University Claude Bernard Lyon 1, Hôpital neurologique-CERMEP, 56 boulevard Pinel, 69394 Lyon cedex 03, France. Received April 6, 2004; revision requested June 18; revision received September 2; accepted October 15. Supported by grants from the Consortium National du Réseau des Génopoles, the European Union Grant Eumorphia QLG2-CT-2002-00930, and the Bonus Quality Recherche of the University Claude Bernard Lyon 1. Address correspondence to C.B. (e-mail: claire.billotey{at}univ-lyon1.fr).

PURPOSE: To evaluate the efficiency of T-cell labeling with anionic magnetic nanoparticles (AMNPs) and in vivo magnetic resonance (MR) imaging monitoring of T-cell homing to the pancreas.

MATERIALS AND METHODS: In vivo MR images of pancreas were obtained with a 7-T MR system in 12 NOD (nonobese diabetic) mice at 11 and 20 days after injection of AMNP-loaded or unloaded T cells. Homing of loaded T cells in pancreatic lymph nodes was detected by the presence of a focal dark spot with T2* effect in a caudal area of the pancreas. Detection of loaded T cells in pancreatic islets was evaluated by comparison of histograms of MR signal intensity generated in whole pancreas in mice injected with loaded and unloaded T cells. Homing of loaded T cells was confirmed at transmission electronic microscopy (TEM). Fifty-six mice underwent all experiments.

RESULTS: Focal dark spots with T2* effect were observed at 11 days in all three mice injected with loaded T cells and in none of the three mice injected with unloaded T cells. At 20 days, a more diffuse negative enhancement of the whole pancreas was noticed in one mouse injected with loaded T cells than in three mice injected with unloaded T cells. Presence of loaded T cells was confirmed with TEM. In vitro and in vivo tests confirmed that survival and function were not altered by loading.

CONCLUSION: The ability of MR imaging to depict cell homing in living organisms at least 20 days after cell labeling was demonstrated, opening the way of follow-up in autoimmune diseases and cell therapy.

© RSNA, 2005






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