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Published online before print March 16, 2006, 10.1148/radiol.2392050277
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(Radiology 2006;239:554-562.)
© RSNA, 2006

Technical Developments

Liver Tumor Model with Implanted Rhabdomyosarcoma in Rats: MR Imaging, Microangiography, and Histopathologic Analysis1

Feng Chen, MD2, Xihe Sun, MD3, Frederik De Keyzer, MSc, Jie Yu, MD, Ronald Peeters, PhD, Walter Coudyzer, MSc, Vincent Vandecaveye, MD, Willy Landuyt, PhD, Hilde Bosmans, PhD, Paul Van Hecke, PhD, Guy Marchal, MD, PhD and Yicheng Ni, MD, PhD

1 From the Department of Radiology (F.C., X.S., F.D.K., J.Y., R.P., W.C., V.V., H.B., P.V.H., G.M., Y.N.) and Laboratory of Experimental Radiobiology and Oncology (W.L.), University Hospitals, Catholic University of Leuven, Herestraat 49, B-3000 Leuven, Belgium. Received February 17, 2005; revision requested April 14; revision received April 25; accepted June 3; final version accepted July 8. Address correspondence to Y.N. (e-mail: Yicheng.Ni{at}med.kuleuven.ac.be).

In compliance with institutional regulations for care and use of laboratory animals, the aim of this study was to establish and characterize a rodent liver tumor model to provide a platform for preclinical assessment of new diagnostic and therapeutic strategies. A rhabdomyosarcoma tumor was implanted in the right and left liver lobes of 20 rats, for a total of 40 tumors. T1- and T2-weighted magnetic resonance (MR) images, diffusion-weighted images, and dynamic susceptibility contrast agent–enhanced perfusion-weighted images were obtained up to 16 days after tumor implantation and were compared with postmortem three-dimensional computed tomographic (CT) images, digital microangiograms, and histopathologic findings. Fifteen tumors were examined with proton (1H) MR spectroscopy. All tumors grew, with a mean volume doubling time of 2.2 days ± 0.9 (standard deviation) and a final size of 591 mm3± 124. The rhabdomyosarcoma tumor showed hypervascularity at MR imaging, three-dimensional CT, microangiography, and histologic analysis. On dynamic susceptibility contrast–enhanced perfusion-weighted images, the maximum signal intensity decrease differed in time and extent between the tumor and the liver, with a significantly (P < .001) higher relative blood volume, relative blood flow, and permeability value in the tumor than in the liver. With 1H MR spectroscopy, the rhabdomyosarcoma tumor and the liver featured significant (P < .001) choline and lipid peaks, respectively. Implantation of a rhabdomyosarcoma tumor in the livers of rats is feasible and reproducible, and this animal model seems promising for future testing of new diagnostic and therapeutic strategies.

© RSNA, 2006






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