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Published online before print December 18, 2008, 10.1148/radiol.2501071929

(Radiology 2009;250:692.)

A more recent version of this article appeared on March 1, 2009
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© RSNA, 2008

Experimental Studies

Mapping the Zonal Organization of Tumor Perfusion and Permeability in a Rat Glioma Model by Using Dynamic Contrast-enhanced Synchrotron Radiation CT1

Daniel Balvay, PhD, Irène Troprès, PhD, Romain Billet, MSc 2, Aurélie Joubert, PhD, Michel Péoc'h, MD, PhD, Charles A. Cuenod, MD, PhD, and Géraldine Le Duc, PhD

1 From the Imaging Research Laboratory, LRI-EA4062 Université Paris Descartes, Paris, France (D.B., C.A.C.); Biomedical Beamline & Biomedical Facility (I.T., R.B., G.L.D.) and INSERM U647, Rayonnement Synchrotron et Recherche Médicale (A.J.), European Synchrotron Radiation Facility, Grenoble, France; IFR1 RMN Biomédicale et Neurosciences, Université Joseph Fourier, Grenoble, France (I.T.); Service d'Anatomie et de Cytologie Pathologique, CHU de Saint-Etienne, Hôpital Bellevue, Saint-Etienne, France (M.P.); and Service de Radiologie, Hôpital Européen G. Pompidou, 20 rue Leblanc, 750015 Paris, France (D.B., C.A.C.). Received November 13, 2007; revision requested February 4, 2008; revision received April 15; accepted July 2; final version accepted July 23. Supported in part by the French Radiology Society (SFR). Address correspondence to D.B. (e-mail: daniel.balvay{at}yahoo.fr).

Purpose: To depict and analyze in vivo the tumor zone organization of C6 gliomas depicted on quantitative parametric maps obtained with dynamic contrast material–enhanced synchrotron radiation computed tomography (CT) in a tightly controlled data-processing protocol.

Materials and Methods: Animal use was compliant with official French guidelines and was assessed by the local Internal Evaluation Committee for Animal Welfare and Rights. Fifteen Wistar rats with orthotopically implanted gliomas were studied at monochromatic synchrotron radiation CT after receiving a bolus injection of contrast material. The iodine concentration maps were analyzed by using a compartmental model selected from among a package of models. Choice of model and assessment of the relevance of the model were guided by quality criteria. Tissue blood flow (FT), tissue blood volume fraction (VT), permeability–surface area product (PS), artery-to-tissue delay (DA-T), and vascular mean transit time (MTT) maps were obtained. Parametric map findings were compared with histologic findings. Local regions of interest were selected in the contralateral hemisphere and in several tumor structures to characterize the tumor microvasculature. Differences in parameter values between regions were assessed with the Wilcoxon method.

Results: Whole-tumor parameters were expressed as means ± standard errors of the mean: Mean FT, VT, PS, and DA-T values and MTT were 61.4 mL/min/100 mL ± 15.3, 2.4% ± 0.4, 0.37 mL/min/100 mL ± 0.11, 0.24 second ± 0.06; and 3.9 seconds ± 0.83, respectively. MTT and mean PS were significantly lower (P < .01) in the normal contralateral tissue: 1.10 seconds ± 0.06 and ≤10–5 mL/min/100 mL, respectively. Tumor regions were characterized by significantly different (P < .05) FT and VT pairs: 108 mL/min/100 mL and 3.66%, respectively, at the periphery; 45.9 mL/min/100 mL and 1.91%, respectively, in the intermediate zone; 5.1 mL/min/100 mL and 0.42%, respectively, in the center; and 210 mL/min/100 mL and 6.82%, respectively, in the maximal value region.

Conclusion: Fine mapping of the glioma microcirculation is feasible with dynamic contrast-enhanced synchrotron radiation CT performed with well-controlled analytic protocols.

Supplemental material: http://radiology.rsnajnls.org/cgi/content/full/2501071929/DC1

© RSNA, 2008