Quantified Power Doppler US of Tumor Blood Flow Correlates with Microscopic Quantification of Tumor Blood Vessels1
Edwin F. Donnelly, MD,
Ling Geng, MD,
Walter E. Wojcicki, MD, PhD,
Arthur C. Fleischer, MD and
Dennis E. Hallahan, MD
1 From the Departments of Radiology (E.F.D., W.E.W., A.C.F.), Radiation Oncology (L.G., D.H.), and Biomedical Engineering (D.H.), Vanderbilt University Medical Center, 1301 22nd Ave S, B-902 The Vanderbilt Clinic, Nashville, TN 37232-5671. Received March 21, 2000; revision requested May 2; revision received July 20; accepted August 29. Supported by National Cancer Institute grants CA58508, CA89674, and CA70937 and a grant from ATL/Phillips. Address correspondence to D.E.H.
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Figure 2a. Representative power Doppler US images from (a) tumors in the control group, (b) tumors treated with TNF, (c) tumors treated with radiation therapy, and (d) tumors receiving combined TNF and radiation therapy. Depicted blood vessels are greatest in the periphery of the tumors (arrowheads in a). Treatment with radiation alone or TNF alone results in a decrease in depicted blood vessels. Combined therapy results in a greater decrease in depicted blood vessels.
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Figure 2b. Representative power Doppler US images from (a) tumors in the control group, (b) tumors treated with TNF, (c) tumors treated with radiation therapy, and (d) tumors receiving combined TNF and radiation therapy. Depicted blood vessels are greatest in the periphery of the tumors (arrowheads in a). Treatment with radiation alone or TNF alone results in a decrease in depicted blood vessels. Combined therapy results in a greater decrease in depicted blood vessels.
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Figure 2c. Representative power Doppler US images from (a) tumors in the control group, (b) tumors treated with TNF, (c) tumors treated with radiation therapy, and (d) tumors receiving combined TNF and radiation therapy. Depicted blood vessels are greatest in the periphery of the tumors (arrowheads in a). Treatment with radiation alone or TNF alone results in a decrease in depicted blood vessels. Combined therapy results in a greater decrease in depicted blood vessels.
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Figure 2d. Representative power Doppler US images from (a) tumors in the control group, (b) tumors treated with TNF, (c) tumors treated with radiation therapy, and (d) tumors receiving combined TNF and radiation therapy. Depicted blood vessels are greatest in the periphery of the tumors (arrowheads in a). Treatment with radiation alone or TNF alone results in a decrease in depicted blood vessels. Combined therapy results in a greater decrease in depicted blood vessels.
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Figure 3. Bar graph shows quantification of power Doppler signal displayed as color area of tumor. Treatment with radiation alone (XRT) or TNF alone results in a decrease in depicted blood vessels. Combined therapy (XRT + TNF) results in a greater decrease in depicted blood vessels. Error bars = SD.
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Figure 4. Bar graph shows the number of vessels per high-power field that are visible with immunofluorescence antibody labeling. A decrease in antibody staining is seen after treatment with either TNF or radiation (XRT) therapy alone. A greater decrease in antibody staining is seen after combination therapy (TNF + XRT). Error bars = SD.
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Figure 5a. Representative photomicrographs of immunofluorescent stained sections of tumors from (a) the control group, (b) the TNF treatment group, (c) the radiation treatment group, and (d) the combination therapy group. A decrease in antibody staining (yellow) is seen after treatment with either TNF or radiation therapy alone. A greater decrease in antibody staining is seen after combination therapy. (Original magnification, x200.)
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Figure 5b. Representative photomicrographs of immunofluorescent stained sections of tumors from (a) the control group, (b) the TNF treatment group, (c) the radiation treatment group, and (d) the combination therapy group. A decrease in antibody staining (yellow) is seen after treatment with either TNF or radiation therapy alone. A greater decrease in antibody staining is seen after combination therapy. (Original magnification, x200.)
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Figure 5c. Representative photomicrographs of immunofluorescent stained sections of tumors from (a) the control group, (b) the TNF treatment group, (c) the radiation treatment group, and (d) the combination therapy group. A decrease in antibody staining (yellow) is seen after treatment with either TNF or radiation therapy alone. A greater decrease in antibody staining is seen after combination therapy. (Original magnification, x200.)
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Figure 5d. Representative photomicrographs of immunofluorescent stained sections of tumors from (a) the control group, (b) the TNF treatment group, (c) the radiation treatment group, and (d) the combination therapy group. A decrease in antibody staining (yellow) is seen after treatment with either TNF or radiation therapy alone. A greater decrease in antibody staining is seen after combination therapy. (Original magnification, x200.)
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Copyright © 2001 by the Radiological Society of North America.
) or with radiation therapy (
) is diminished relative to that in the control group (
). Tumors receiving combined treatment (
) decreased in size over time. Error bars = SD.