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Excised Bone Structures in Mice: Imaging at Three-dimensional Synchrotron Radiation Micro CT¹

¹ From the European Synchrotron Radiation Facility, BP 220, 38043 Grenoble, France (E.M.B., S.N., A.E., F.P.); LBTO, Equipe Mixte, Institut National de la Santé et de la Recherche Médicale, E9901 Faculté de Médecine, Saint-Etienne, France (D.A., L.V.); and CREATIS, UMR 5515 Centre National de la Recherche Scientifique, Villeurbanne, France (S.N., A.E., F.P.). Received May 10, 2002; revision requested July 25; final revision received February 26, 2003; accepted April 30. Supported by a Marie Curie Fellowship of the European Community program Human Potential under contract number HPMF-CT-1999–00194 and by grants from the European Research in Space and Terrestrial Osteoporosis group and Région Rhône-Alpes. Address correspondence to F.P. (peyrin@esrf.fr).

View larger version (19K): [in a new window]   Figure 1. SR micro CT experimental setup. Different elements of 3D acquisition device from left to right: monochromatic x-ray beam extracted from SR, sample stage, and 2D detector.

View larger version (112K): [in a new window]   Figure 2. Representative images of C3H mouse. A, Mediolateral radiograph shows distal part of the femur. B, Posteroanterior 3D reconstructed volume shows selected region of distal part of the femur to be analyzed.

View larger version (68K): [in a new window]   Figure 3. Images show 3D displays of ROIs extracted from reconstructed bone volume. Left column: Images in C3H mice. Right column: Images in B6 mice. A, B, Trabecular ROIs. C, D, Cortical ROIs. E, F, Pores in cortical ROIs.

View larger version (26K): [in a new window]   Figure 4. Box plots for trabecular bone mass and microarchitectural parameters (all estimated from 3D measurements). A, Ratio of bone volume to total tissue volume (BV/TV*). B, Ratio of bone surface to bone volume (BS/BV*). C, Trabecular thickness (Tb.Th*). D, Trabecular separation (Tb.Sp*). E, Trabecular number (Tb.N*). F, Trabecular connectivity density (ConnD*). G, Degree of anisotropy (DA). Horizontal lines in each box from top to bottom indicate 25th, 50th, and 75th percentiles. Error bars indicate 10th and 90th percentiles; dots correspond to minimum and maximum values. Dagger denotes that differences between C3H and B6 strains are significant (P < .05).

View larger version (15K): [in a new window]   Figure 5. Two-dimensional plots of cortical parameters in C3H () and B6 () mice. Two strains of mice can be differentiated when they are described with a pair of cortical parameters (all estimated from 3D measurements). A, Cortical thickness (Ct.Th*) and cortical porosity (Ct.Por*). B, Relative cortical volume, expressed as the ratio of cortical bone volume to total tissue volume (CtV/TV*), and cortical connectivity density (Ct.ConnD*).

View larger version (32K): [in a new window]   Figure 6. Graph shows differences between C3H and B6 strains of mice in terms of mineralization. Mean histogram of each strain is plotted: Solid curves correspond to C3H strain; and dotted curves, to B6 strain. Dark curves correspond to trabecular (trab) ROIs; and light curves, to cortical (cort) ROIs.