Published online before print April 21, 2005, 10.1148/radiol.2353040726
Cytotoxic Effects of Ionic High-osmolar, Nonionic Monomeric, and Nonionic Iso-osmolar Dimeric Iodinated Contrast Media on Renal Tubular Cells in Vitro1
Marc C. Heinrich, MD,
Martin K. Kuhlmann, MD,
Aleksandar Grgic, MD,
Martina Heckmann, MD,
Bernhard Kramann, MD and
Michael Uder, MD
1 From the Department of Diagnostic Radiology (M.C.H., A.G., M.H., B.K.) and Department of Medicine, Division of Nephrology and Hypertension (M.K.K.), University Hospital of Saarland, Homburg/Saar, Germany; and Department of Diagnostic Radiology, University Hospital of Erlangen, Maximiliansplatz 1, 91054 Erlangen, Germany (M.U.). From the 2004 RSNA Annual Meeting. Received April 22, 2004; revision requested June 29; revision received July 21; accepted August 18. Address correspondence to M.U. (e-mail: michael.uder@idr.imed.uni-erlangen.de).
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Figure 1. Graph shows concentration and time dependency of the cytotoxic effect of iomeprol-300 in LLC-PK1 cells as assessed with MTT assay. The cells were incubated with iomeprol-300 for 1, 5, and 24 hours, respectively, at concentrations ranging from 18.75 to 150 mg of iodine per milliliter (mg I/ml). The data were reported as percentage of undamaged control cells (% of control). Data show the mean ± standard error of the mean (n = 12).
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Figure 2. Graph shows comparison of cytotoxic effects of different iodinated contrast media as assessed with MTT assay at equal iodine concentrations. LLC-PK1 cells were incubated with contrast media for 24 hours at concentrations of 37.5 and 75 mg of iodine per milliliter (mg I/ml). Ioxithalamate induced a significantly stronger effect than did the other contrast media. There was no significant difference between the nonionic contrast media. The data were reported as percentage of undamaged control cells (% of control). Data show the mean ± standard error of the mean (n = 61-90). * = P < .001 for ioxithalamate versus iodixanol, ioxithalamate versus iotrolan, ioxithalamate versus iomeprol-300, and ioxithalamate versus ioversol.
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Figure 3. Graph shows cytotoxic effects of different iodinated contrast media assessed with MTT assay plotted against the concentration of contrast media molecules in the solution (millimoles of contrast media per liter). LLC-PK1 cells were incubated with contrast media for 24 hours at concentrations ranging from 49.25 to 197 mmol/L (mM). The dimeric contrast media iotrolan and iodixanol showed a significantly stronger inhibition of MTT conversion than did the monomeric contrast media ioversol and iomeprol-300. The data were reported as percentage of undamaged control cells (% of control). Data show the mean ± standard error of the mean (n = 61-90). * = P < .001 for ioxithalamate versus ioversol and ioxithalamate versus iomeprol-300; + = P < .001 for ioversol versus iodixanol, ioversol versus iotrolan, iomeprol-300 versus iodixanol, and iomeprol-300 versus iotrolan.
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Figure 4. Comparison of cytotoxic effects of ioxithalamate and iomeprol-300 with equal volumes of isotonic saline and equiosmolal mannitol solutions as assessed with MTT assay. LLC-PK1 cells were incubated with contrast media, 0.9% NaCl, and corresponding mannitol solutions, respectively, for 24 hours at concentrations of 12.5% and 25% vol/vol. Dilution of the growth media with 0.9% NaCl showed only minor effects. The effects of the mannitol solutions were significantly weaker than those of the corresponding contrast media. The data were reported as percentage of undamaged control cells (% of control). Data show the mean ± standard error of the mean (n = 54-90). * = P < .001 for mannitol-520 versus iomeprol-300 and mannitol-1860 versus ioxithalamate, mg I/ml = milligrams of iodine per milliliter.
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Figure 5. Graph shows comparison of cytotoxic effects of the dimeric iso-osmolar contrast media iotrolan and iodixanol with those of low-osmolar monomer iomeprol-300 and those of the iso-osmolar formulation iomeprol-150 assessed with MTT assay at equimolar concentrations (in millimoles per liter [mM]). LLC-PK1 cells were incubated with contrast media for 24 hours at concentrations ranging from 49.25 to 197 mmol/L. Dimeric contrast media iotrolan and iodixanol showed a significantly stronger inhibition of MTT conversion than did monomeric contrast media iomeprol-300 and iomeprol-150. Iomeprol-150 induced a slightly weaker effect than did iomeprol-300. The data were reported as percentage of undamaged control cells (% of control). Data show the mean ± standard error of the mean (n = 17-40). * = P < .001 for iodixanol versus iomeprol-300, iodixanol versus iomeprol-150, iotrolan versus iomeprol-300, and iotrolan versus iomeprol-150; + = P < .05 for iomeprol-150 versus iomeprol-300.
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Copyright © 2005 by the Radiological Society of North America.
