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Figure 4a: Color plot of the orientation of the eigenvectors, for the mouse and image section shown in Figure 2, (a) immediately after femoral artery ligation; (b) 3 days after ligation, when all eigenvalues were increased; (c) 10 days after ligation; and (d) 21 days after ligation. A change in eigenvector orientation after 3 days was observed in half the mice. The effects were more pronounced for some animals, which had more pixels with changed orientation. At day 10, a region of changed orientation was still present in the gastrocnemius. This region corresponded to the location of increased ADC (Fig 2). The eigenvector orientations were color coded as follows: Blue indicated out of plane; green, top to bottom; and red, left to right.