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Figure 7: Micrographs of representative infarcts in, A, C, E, control animals and, B, D, F, AAV-VEGF–treated animals. A, B, At low magnification, infarct in control animal shows no appreciable neovascularization. A, Remodeled thick-walled blood vessels (arrows). B, AAV-VEGF–treated animal contains numerous vessels (arrowheads) in linear array representing injection needle track. C, D, At high magnification, control infarct has a few scattered remodeled thick-walled vessels (arrows), while AAV-VEGF–treated infarct contains numerous thin-walled vessels (arrowheads) filled with blood, suggesting active blood vessels. E, F, Biotinylated isolectin B4 stain localizes all vessels with brown reaction product. Sparse vessels in control infarct (arrows) and numerous vessels in AAV-VEGF–treated infarct (arrowheads) were demonstrated with lectin stain. I = infarct, LV = left ventricular cavity. A, B, Calibration bars = 1000 µm. C, D, E, F, Calibration bars = 200 µm. (A, B, C, D, Masson trichrome stain; E, F, immunoperoxidase methods with biotinylated isolectin B4 stain.)
