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Figure 3: Detected iron oxides in monocytes with Prussian blue staining. Iron oxide is blue, and cell nuclei are red. A, Vehicle-treated monocytes. B, Monocytes incubated with USPIOs at a concentration of 1.0 mg Fe/mL. C, Monocytes incubated with SPIOs at a concentration of 1.0 mg Fe/mL. D, Higher magnification of SPIO-labeled monocytes. (Prussian blue stain; original magnification, x40 [AC], x100 [D].)