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Figure 6b: Electron microscopic investigation of (a, b) transfected and (c–e) nontransfected cells treated with Resovist (R). (a, b) Cells transfected (with jetPEI) were not able to ingest the iron-containing Resovist at 4 hours incubation. Resovist remains on the cellular surface. (c–e) Nontransfected cells showed a consistent and time-dependent uptake of Resovist. In c (15 hours incubation), * shows phagocytic vacuole; this detail is shown at greater magnification in insert. In d (15 hours incubation), the Resovist-containing vacuole lies directly beneath both an extracellular deposit of Resovist and a cistern of rough endoplasmic reticulum (rER), indicating a high rate of protein synthesis in this cell. The dynamic process of Resovist incubation is demonstrated in e (4 hours incubation), which shows extracellular Resovist and some uptaken Resovist already. Black arrows show Resovist, which was taken up by the cells. White arrows show heterochromatin. N = nucleus. Bars in a, b, c, and e = 1 µm; bar in d = 0.5 µm.
