| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Experimental Studies |
1 From Dept of Radiology (H.E.D.L., M.S., S.M., E.J.R., T.M.L.), Inst of Pathology (M.R., G.P., U.H., J.S.), Third Clinic of Internal Medicine, Laboratory of Stem Cell Physiology (R.A.J.O., U.K.), Dept Clinical Chemistry and Pathochemistry (H.R.), and National Research Ctr for Environment and Health (U.H.), Technical Univ, Ismaninger Str 22, 81675 Munich, Germany. Received Apr 2, 2002; revision requested Jun 13; final revision received Oct 31; accepted Jan 14, 2003. Supported by German Research Foundation grant DA 529/1-1. Address correspondence to H.E.D.L. (e-mail: daldrup@roe.med.tu-muenchen.de).
PURPOSE: To label human hematopoietic progenitor cells with various magnetic resonance (MR) imaging contrast agents and to obtain 1.5-T MR images of them.
MATERIALS AND METHODS: Hematopoietic progenitor cells, labeled with ferumoxides, ferumoxtran, magnetic polysaccharide nanoparticles–transferrin, P7228 liposomes, and gadopentetate dimeglumine liposomes underwent MR imaging with T1- and T2-weighted spin-echo and fast field-echo sequences. Data were analyzed by measuring MR signal intensities and R1 and R2* relaxation rates of labeled cells and nonlabeled control cells. Mean quantitative data for the various contrast agent groups were assessed for significant differences compared with control cells by means of the Scheffe test. As a standard of reference, MR imaging data were compared with electron microscopic and spectrometric data.
RESULTS: For all contrast agents, intracellular cytoplasm uptake was demonstrated with electron microscopy and was quantified with spectrometry. When compared with nonlabeled control cells, progenitor cells labeled with iron oxides showed significantly (P < .05) increased R2*. Cells labeled with gadopentetate dimeglumine liposomes showed significantly increased R1. Detection thresholds were 5 x 105 cells for gadopentetate dimeglumine liposomes and ferumoxtran, 2.5 x 105 cells for ferumoxides and P7228 liposomes, and 1 x 105 cells for magnetic polysaccharide nanoparticles–transferrin.
CONCLUSION: Hematopoietic progenitor cells can be labeled with MR contrast agents and can be depicted with a standard 1.5-T MR imager.
© RSNA, 2003
Index terms: Experimental study • Iron • Magnetic resonance (MR), contrast media • Magnetic resonance (MR), experimental studies, 57.121411, 57.121413 • Molecular analysis • Stem cells
This article has been cited by other articles:
|
|
 |
|
  G. Bierry, F. Jehl, N. Boehm, P. Robert, G. Prevost, J.-L. Dietemann, H. Desal, and S. Kremer Macrophage Activity in Infected Areas of an Experimental Vertebral Osteomyelitis Model: USPIO-enhanced MR Imaging--Feasibility Study Radiology, July 1, 2008; 248(1): 114 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Maxwell, J. Bonde, D. A. Hess, S. A. Hohm, R. Lahey, P. Zhou, M. H. Creer, D. Piwnica-Worms, and J. A. Nolta Fluorophore-Conjugated Iron Oxide Nanoparticle Labeling and Analysis of Engrafting Human Hematopoietic Stem Cells Stem Cells, February 1, 2008; 26(2): 517 - 524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kuhlpeter, H. Dahnke, L. Matuszewski, T. Persigehl, A. von Wallbrunn, T. Allkemper, W. L. Heindel, T. Schaeffter, and C. Bremer R2 and R2* Mapping for Sensing Cell-bound Superparamagnetic Nanoparticles: In Vitro and Murine in Vivo Testing Radiology, November 1, 2007; 245(2): 449 - 457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ju, G.-J. Teng, H. Lu, Y. Zhang, A. Zhang, F. Chen, and Y. Ni In Vivo MR Tracking of Mesenchymal Stem Cells in Rat Liver after Intrasplenic Transplantation Radiology, October 1, 2007; 245(1): 206 - 215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Oude Engberink, S. M. A. van der Pol, E. A. Dopp, H. E. de Vries, and E. L. A. Blezer Comparison of SPIO and USPIO for in Vitro Labeling of Human Monocytes: MR Detection and Cell Function Radiology, May 1, 2007; 243(2): 467 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-C. Shyu, C.-P. Chen, S.-Z. Lin, Y.-J. Lee, and H. Li Efficient Tracking of Non-Iron-Labeled Mesenchymal Stem Cells With Serial MRI in Chronic Stroke Rats Stroke, February 1, 2007; 38(2): 367 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Dousset, B. Brochet, M.S.A. Deloire, L. Lagoarde, B. Barroso, J.-M. Caille, and K.G. Petry MR Imaging of Relapsing Multiple Sclerosis Patients Using Ultra-Small-Particle Iron Oxide and Compared with Gadolinium. AJNR Am. J. Neuroradiol., May 1, 2006; 27(5): 1000 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Matuszewski, T. Persigehl, A. Wall, W. Schwindt, B. Tombach, M. Fobker, C. Poremba, W. Ebert, W. Heindel, and C. Bremer Cell Tagging with Clinically Approved Iron Oxides: Feasibility and Effect of Lipofection, Particle Size, and Surface Coating on Labeling Efficiency Radiology, April 1, 2005; 235(1): 155 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. H. Kim, D. S. Lee, J. H. Kang, Y. J. Lee, J.-K. Chung, J.-K. Roh, S. U. Kim, and M. C. Lee Reversing the Silencing of Reporter Sodium/Iodide Symporter Transgene for Stem Cell Tracking J. Nucl. Med., February 1, 2005; 46(2): 305 - 311. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. E. Daldrup-Link, M. Rudelius, G. Piontek, S. Metz, R. Brauer, G. Debus, C. Corot, J. Schlegel, T. M. Link, C. Peschel, et al. Migration of Iron Oxide-labeled Human Hematopoietic Progenitor Cells in a Mouse Model: In Vivo Monitoring with 1.5-T MR Imaging Equipment Radiology, January 1, 2005; 234(1): 197 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dolovich and R. Labiris Imaging Drug Delivery and Drug Responses in the Lung Proceedings of the ATS, December 1, 2004; 1(4): 329 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Bos, Y. Delmas, A. Desmouliere, A. Solanilla, O. Hauger, C. Grosset, I. Dubus, Z. Ivanovic, J. Rosenbaum, P. Charbord, et al. In Vivo MR Imaging of Intravascularly Injected Magnetically Labeled Mesenchymal Stem Cells in Rat Kidney and Liver Radiology, December 1, 2004; 233(3): 781 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. V. Frangioni and R. J. Hajjar In Vivo Tracking of Stem Cells for Clinical Trials in Cardiovascular Disease Circulation, November 23, 2004; 110(21): 3378 - 3383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. S. Arbab, G. T. Yocum, H. Kalish, E. K. Jordan, S. A. Anderson, A. Y. Khakoo, E. J. Read, and J. A. Frank Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI Blood, August 15, 2004; 104(4): 1217 - 1223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A S K Dzik-Jurasz Molecular imaging in vivo: an introduction Br. J. Radiol., December 1, 2003; 76(suppl_2): S98 - S109. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| RADIOLOGY | RADIOGRAPHICS | RSNA JOURNALS ONLINE |
