HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text All Versions of this Article: 2353040726v1 235/3/843    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Heinrich, M. C. Articles by Uder, M. Search for Related Content PubMed PubMed Citation Articles by Heinrich, M. C. Articles by Uder, M.

Cytotoxic Effects of Ionic High-osmolar, Nonionic Monomeric, and Nonionic Iso-osmolar Dimeric Iodinated Contrast Media on Renal Tubular Cells in Vitro¹

¹ From the Department of Diagnostic Radiology (M.C.H., A.G., M.H., B.K.) and Department of Medicine, Division of Nephrology and Hypertension (M.K.K.), University Hospital of Saarland, Homburg/Saar, Germany; and Department of Diagnostic Radiology, University Hospital of Erlangen, Maximiliansplatz 1, 91054 Erlangen, Germany (M.U.). From the 2004 RSNA Annual Meeting. Received April 22, 2004; revision requested June 29; revision received July 21; accepted August 18. Address correspondence to M.U. (e-mail: michael.uder@idr.imed.uni-erlangen.de).

PURPOSE: To compare the cytotoxic effects of dimeric and monomeric iodinated contrast media on renal tubular cells in vitro with regard to osmolality.

MATERIALS AND METHODS: LLC-PK1 cells were incubated with ioxithalamate, ioversol, iomeprol-300, iomeprol-150, iodixanol, iotrolan, and hyperosmolar mannitol solutions for 1–24 hours at concentrations from 18.75 to 150 mg of iodine per milliliter. Cytotoxic effects were assessed with 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Data were analyzed with one-way analysis of variance; post hoc tests were performed.

RESULTS: At equal iodine concentrations, ioxithalamate showed stronger cytotoxic effects than did other contrast media (MTT conversion for ioxithalamate was 4% vs that for ioversol of 32%, that for iomeprol-300 of 34%, that for iodixanol of 40%, and that for iotrolan of 41% of undamaged control cells at 75 mg of iodine per milliliter, n = 61–90, P < .001); there was no significant difference between low-osmolar monomeric and iso-osmolar dimeric contrast media (P > .05). At equal molarity, dimeric contrast media induced significantly stronger cytotoxic effects than did low-osmolar monomeric contrast media (40% for iodixanol and 41% for iotrolan vs 64% for ioversol and 59% for iomeprol-300 at 98.5 mmol/L, n = 61–75, P < .001). At equimolar concentrations, both dimeric contrast media showed stronger cytotoxic effects than did iso-osmolar formulation of iomeprol-150 (51% for iodixanol and 50% for iotrolan vs 77% for iomeprol-150 at 98.5 mmol/L, n = 35–40, P < .001). Mannitol solutions induced weaker cytotoxic effects than did corresponding contrast media compounds (74% for mannitol-520 vs 34% for iomeprol-300 and 41% for mannitol-1860 vs 4% for ioxithalamate, P < .001).

CONCLUSION: Besides hyperosmolality, direct cytotoxic effects of contrast media molecules contribute to their cytotoxic effects. Results of this study indicate that dimeric contrast media molecules have a greater potential for cytotoxic effects on proximal renal tubular cells in vitro than do monomeric contrast media molecules.

© RSNA, 2005

This article has been cited by other articles:

				M. Treitl, H. Rupprecht, S. Wirth, M. Korner, M. Reiser, and J. Rieger Assessment of renal vasoconstriction in vivo after intra-arterial administration of the isosmotic contrast medium iodixanol compared to the low-osmotic contrast medium iopamidol Nephrol. Dial. Transplant., November 25, 2008; (2008) gfn638v1. [Abstract] [Full Text] [PDF]

				R. C. Harmon, S. P. Duffy, M. V. Terneus, J. G. Ball, and M. A. Valentovic Characterization of a novel model for investigation of radiocontrast nephrotoxicity Nephrol. Dial. Transplant., October 7, 2008; (2008) gfn540v1. [Abstract] [Full Text] [PDF]

				G. Romano, C. Briguori, C. Quintavalle, C. Zanca, N. V. Rivera, A. Colombo, and G. Condorelli Contrast agents and renal cell apoptosis Eur. Heart J., October 2, 2008; 29(20): 2569 - 2576. [Abstract] [Full Text] [PDF]

				S. A. Nguyen, P. Suranyi, J. G. Ravenel, P. K. Randall, P. B. Romano, K. A. Strom, P. Costello, and U. J. Schoepf Iso-Osmolality versus Low-Osmolality Iodinated Contrast Medium at Intravenous Contrast-enhanced CT: Effect on Kidney Function Radiology, July 1, 2008; 248(1): 97 - 105. [Abstract] [Full Text] [PDF]

				B. Elmstahl, U. Nyman, P. Leander, K. Golman, C.-M. Chai, D. Grant, R. Doughty, R. Pehrson, J. Bjork, and T. Almen Iodixanol 320 Results in Better Renal Tolerance and Radiodensity than Do Gadolinium-based Contrast Media: Arteriography in Ischemic Porcine Kidneys Radiology, April 1, 2008; 247(1): 88 - 97. [Abstract] [Full Text] [PDF]

				E. Seeliger, B. Flemming, T. Wronski, M. Ladwig, K. Arakelyan, M. Godes, M. Mockel, and P. B. Persson Viscosity of Contrast Media Perturbs Renal Hemodynamics J. Am. Soc. Nephrol., November 1, 2007; 18(11): 2912 - 2920. [Abstract] [Full Text] [PDF]

				G. T. C. Wong and M. G. Irwin Contrast-induced nephropathy Br. J. Anaesth., October 1, 2007; 99(4): 474 - 483. [Abstract] [Full Text] [PDF]

				M. C. Heinrich, M. K. Kuhlmann, S. Kohlbacher, M. Scheer, A. Grgic, M. B. Heckmann, and M. Uder Cytotoxicity of Iodinated and Gadolinium-based Contrast Agents in Renal Tubular Cells at Angiographic Concentrations: In Vitro Study Radiology, December 19, 2006; (2006) 2422060245. [Abstract] [Full Text]

				M. O. Downes, M. Heinrich, and M. Uder In vitro study of various iodinated contrast media. Radiology, June 1, 2006; 239(3): 918 - 921. [Full Text] [PDF]

				H. T. Lee, M. Jan, S. C. Bae, J. D. Joo, F. R. Goubaeva, J. Yang, and M. Kim A1 adenosine receptor knockout mice are protected against acute radiocontrast nephropathy in vivo Am J Physiol Renal Physiol, June 1, 2006; 290(6): F1367 - F1375. [Abstract] [Full Text] [PDF]

				M. Tepel, P. Aspelin, and N. Lameire Contrast-Induced Nephropathy: A Clinical and Evidence-Based Approach Circulation, April 11, 2006; 113(14): 1799 - 1806. [Full Text] [PDF]

				T. Skotland, D. Grant, M. Heinrich, and M. Uder In Vitro Cytotoxic Effects of Iodinated Contrast Media on a Renal Tubular Cell Line Radiology, February 1, 2006; 238(2): 758 - 760. [Full Text] [PDF]

				M. Heinrich, M. Uder, and T. Gleeson Pathogenesis of Contrast-Induced Nephropathy Am. J. Roentgenol., October 1, 2005; 185(4): 1079 - 1079. [Full Text] [PDF]

				R. W. Katzberg Contrast Medium-induced Nephrotoxicity: Which Pathway? Radiology, June 1, 2005; 235(3): 752 - 755. [Full Text] [PDF]